APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human VCP.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
valosin containing protein , CDC48 , TERA , p97
Cellular Localization
Cytoplasm, cytosol,Endoplasmic reticulum,Nucleus
Background
This gene encodes a member of the AAA ATPase family of proteins. The encoded protein plays a role in protein degradation, intracellular membrane fusion, DNA repair and replication, regulation of the cell cycle, and activation of the NF-kappa B pathway. This protein forms a homohexameric complex that interacts with a variety of cofactors and extracts ubiquitinated proteins from lipid membranes or protein complexes. Mutations in this gene cause IBMPFD (inclusion body myopathy with paget disease of bone and frontotemporal dementia), ALS (amyotrophic lateral sclerosis) and Charcot-Marie-Tooth disease in human patients. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
|
GTX640431 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [HL3018] (GTX640431) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX640431 WB Image
Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [HL3018] (GTX640431) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX640431 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [HL3018] (GTX640431) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX640431 IHC-P Image
VCP antibody [HL3018] detects VCP protein by immunohistochemical analysis.Sample: Paraffin-embedded human oral carcinoma.VCP stained by VCP antibody [HL3018] (GTX640431) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX640431 IHC-P Image
VCP antibody [HL3018] detects VCP protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.VCP stained by VCP antibody [HL3018] (GTX640431) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX640431 IHC-P Image
VCP antibody [HL3018] detects VCP protein by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.VCP stained by VCP antibody [HL3018] (GTX640431) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX640431 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [HL3018] (GTX640431) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX640431 ICC/IF Image
VCP antibody [HL3018] detects VCP protein by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VCP stained by VCP antibody [HL3018] (GTX640431) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
|
REFERENCE
There are currently no references for VCP antibody [HL3018] (GTX640431). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for VCP antibody [HL3018] (GTX640431). Be the first to share your experience with this product.