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VCP antibody [HL3018]

Recombinant antibody
Anti-VCP antibody [HL3018] used in Western Blot (WB). GTX640431
Anti-VCP antibody [HL3018] used in Western Blot (WB). GTX640431
Anti-VCP antibody [HL3018] used in Western Blot (WB). GTX640431
Anti-VCP antibody [HL3018] used in IHC (Paraffin sections) (IHC-P). GTX640431
Anti-VCP antibody [HL3018] used in IHC (Paraffin sections) (IHC-P). GTX640431
Anti-VCP antibody [HL3018] used in IHC (Paraffin sections) (IHC-P). GTX640431
Anti-VCP antibody [HL3018] used in Western Blot (WB). GTX640431
Anti-VCP antibody [HL3018] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640431

Cat. No. GTX640431

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL3018

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all VCP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human VCP.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

valosin containing protein , CDC48 , TERA , p97

Cellular Localization

Cytoplasm, cytosol,Endoplasmic reticulum,Nucleus

Background

This gene encodes a member of the AAA ATPase family of proteins. The encoded protein plays a role in protein degradation, intracellular membrane fusion, DNA repair and replication, regulation of the cell cycle, and activation of the NF-kappa B pathway. This protein forms a homohexameric complex that interacts with a variety of cofactors and extracts ubiquitinated proteins from lipid membranes or protein complexes. Mutations in this gene cause IBMPFD (inclusion body myopathy with paget disease of bone and frontotemporal dementia), ALS (amyotrophic lateral sclerosis) and Charcot-Marie-Tooth disease in human patients. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-VCP antibody [HL3018] used in Western Blot (WB). GTX640431

GTX640431 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [HL3018] (GTX640431) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody [HL3018] used in Western Blot (WB). GTX640431

GTX640431 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [HL3018] (GTX640431) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody [HL3018] used in Western Blot (WB). GTX640431

GTX640431 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [HL3018] (GTX640431) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody [HL3018] used in IHC (Paraffin sections) (IHC-P). GTX640431

GTX640431 IHC-P Image

VCP antibody [HL3018] detects VCP protein by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
VCP stained by VCP antibody [HL3018] (GTX640431) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VCP antibody [HL3018] used in IHC (Paraffin sections) (IHC-P). GTX640431

GTX640431 IHC-P Image

VCP antibody [HL3018] detects VCP protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
VCP stained by VCP antibody [HL3018] (GTX640431) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VCP antibody [HL3018] used in IHC (Paraffin sections) (IHC-P). GTX640431

GTX640431 IHC-P Image

VCP antibody [HL3018] detects VCP protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
VCP stained by VCP antibody [HL3018] (GTX640431) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VCP antibody [HL3018] used in Western Blot (WB). GTX640431

GTX640431 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [HL3018] (GTX640431) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody [HL3018] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640431

GTX640431 ICC/IF Image

VCP antibody [HL3018] detects VCP protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VCP stained by VCP antibody [HL3018] (GTX640431) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations